Composite

Part:BBa_J36844:Design

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-30)


Lpp-OmpA(46-66)-Streptavidin wild-type + His6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 248
    Illegal AgeI site found at 299
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.


Source

Assembly of J36835, J36836, and J36841

References